Application

High-performance liquid chromatography (HPLC) is a separation and detection technology based on classic liquid chromatography and combined with gas chromatography. It has the characteristics of efficient detection speed, automated operation, and wide detection range. HPLC is widely used in the detection of nutrients, additives, pesticide and veterinary drug residues in food. Regular maintenance of high-performance liquid chromatography can ensure that the performance of the instrument and equipment is under control, thereby ensuring the accuracy and effectiveness of test results.

High performance liquid chromatography usually consists of a high-pressure infusion pump, an automatic sampler, a chromatographic column, and a detector. High-performance liquid chromatography uses microporous particles as the stationary phase, which is filled in a small and short stainless steel column. The mobile phase (single solvents with different polarities, mixed solvents with different ratios/volumes, buffers, etc.) passes through a high-pressure infusion pump. Entering a chromatographic column with high column pressure greatly accelerates the diffusion rate of solutes in the stationary phase, which can achieve the effects of short analysis time, high column efficiency, and efficient separation capability. The separated solutes enter the detector and ultimately achieve the analysis and detection of the sample.